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reuteri strain (ATCC 23272, GDMCC) was cultured in a sealed centrifuge tube containing de Man, Rogosa, and Sharpe (MRS) broth (HB0384, Hopebio, China) or in a carbon dioxide-generating bag containing MRS agar medium to maintain a microaerophilic environment at 37 °C

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Lower Pituitary Signaling The hypothalamus–pituitary–gonadal (HPG) axis becomes less efficient, reducing the signal for testosterone production