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TUNEL Cell or tissue sections were fixed with 4% paraformaldehyde at room temperature for 15 min, followed by three washes with phosphate-buffered saline (PBS)

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Comparable to the special controls for device classification under 21 CFR 882.5801, Computerized behavioral therapy device for psychiatric disorders, the 21 CFR 882.5803 device classification's special controls require the use of a validated measure to evaluate effectiveness of the device to provide therapy for ADHD or any of its individual symptoms